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Journal / Biology / Isolering av enteriska gliaceller från Submucosa…
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Isolering av enteriska gliaceller från Submucosa och Lamina Propria av vuxen musen
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Isolering av enteriska gliaceller från Submucosa och Lamina Propria av vuxen musen

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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Här beskriver vi isolering av enteriska-glial celler från intestinal-submucosa använda sekventiell EDTA inkubationer för att kelat divalenta katjoner och sedan inkubation i icke-enzymatiska cell återställningslösning. Plätering resulterande cellsuspension på poly-D-lysin och laminin resulterar i en höganrikat kultur av submukösa gliaceller för funktionell analys.

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Biologi fråga 138 tolvfingertarmen hGFAP-Cre tdTomato EDTA kelering EGCs Ca2 + flux Sox10 Fredsgenomförande
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