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Journal / Biology / Submukoza ve Lamina Propria yetişkin fare enterik …
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Submukoza ve Lamina Propria yetişkin fare enterik gliyal hücrelerini izolasyon
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Submukoza ve Lamina Propria yetişkin fare enterik gliyal hücrelerini izolasyon

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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Burada, bağırsak submukoza divalent katyonlar ve enzimatik olmayan hücre kurtarma çözümü kuluçka şelasyon yapın için sıralı EDTA incubations kullanarak gliyal enterik hücre izolasyon açıklayın. Sonuç hücre süspansiyon poli-D-lizin ve laminin kaplama submucosal Gliyal hücreler fonksiyonel analiz için zenginleştirilmiş bir kültür sonuçlanır.

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Biyoloji sayı 138 oniki parmak bağırsağı hGFAP-Cre tdTomato EDTA şelasyon EGCs Ca2 + akı Sox10 GFAP'nin
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