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Micromanipulation technieken waardoor analyse van morfogenetische dynamiek en een omzet van Cytoskeletal regelgevers
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Micromanipulation technieken waardoor analyse van morfogenetische dynamiek en een omzet van Cytoskeletal regelgevers

Article DOI: 10.3791/57643-v • 12:52 min • May 12th, 2018
May 12th, 2018 •
1,2, 1,2
1Division of Molecular Cell Biology, Zoological Institute, Technische Universität Braunschweig, 2Department of Cell Biology, Helmholtz Centre for Infection Research

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We beschrijven hoe micro- en photomanipulation technieken zoals FRAP en photoactivation inschakelen de bepaling van de parameters van de beweeglijkheid en de spatio dynamiek van eiwitten in het migreren van cellen. Experimentele uitlezingen omvatten subcellular dynamiek en omzet van beweeglijkheid toezichthouders of van de onderliggende actine cytoskelet.

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Biologie kwestie 135 Microinjection FRAP photoactivation PA-GFP met actine lamellipodium cytoskelet migratie
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