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Journal / Neuroscience / Visualizzazione dei depositi β amiloide nel…
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Neuroscience

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JoVE Journal Neuroscience
Visualizzazione dei depositi β amiloide nel cervello umano con Matrix-assisted Laser Desorption/Ionization Imaging spettrometria di massa
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Visualizzazione dei depositi β amiloide nel cervello umano con Matrix-assisted Laser Desorption/Ionization Imaging spettrometria di massa

Article DOI: 10.3791/57645-v • 09:31 min • March 7th, 2019
March 7th, 2019 •
*1, *2, 1, 1, 1, 3, 4
1Department of Life and Medical Systems, Doshisha University, 2Bruker Daltonics K.K., 3The Brain Bank for Aging Research, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 4Graduate School of Brain Science, Doshisha University
* These authors contributed equally

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Imaging molecolare con matrix-assisted laser desorbimento/ionizzazione-based imaging spettrometria di massa (MALDI-IMS) consente la mappatura simultanea di più analiti in campioni biologici. Qui, presentiamo un protocollo per la rilevazione e visualizzazione della proteina β amiloide sui tessuti di cervello del morbo di Alzheimer e campioni di angiopatia amiloide cerebrale usando MALDI-IMS.

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Neuroscienze problema 145 spettrometria di massa MALDI imaging cervello umano autopsia β amiloide malattia di Alzheimer placche senili Angiopatia amiloide cerebrale
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