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Journal / Biochemistry / Rilevazione di piccolo GTPase prenilazione e GTP…
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Biochemistry
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JoVE Journal Biochemistry
Rilevazione di piccolo GTPase prenilazione e GTP Binding mediante frazionamento di membrana e GTPase-collegata dell'immunosorbente
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
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Rilevazione di piccolo GTPase prenilazione e GTP Binding mediante frazionamento di membrana e GTPase-collegata dell'immunosorbente

Article DOI: 10.3791/57646-v • 13:52 min • November 11th, 2018
November 11th, 2018 •
1,2, 1,2,3, 1, 1,2, 4, 5, 6,7,8, 1,9, 1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

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Qui descriviamo un protocollo per studiare la prenilazione e guanosina-5'-trifosfato (GTP)-caricamento di Rho GTPasi. Questo protocollo è costituito da due metodi dettagliati, vale a dire del kit membrana frazionamento e un GTPase-collegata dell'immunosorbente. Il protocollo può essere utilizzato per misurare la prenilazione e GTP caricamento di diverse altre piccole GTPasi.

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Biochimica problema 141 simvastatina glioblastoma Rho GTPasi biologia delle cellule di cancro prenylation della via di acido mevalonico
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