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Journal / Biochemistry / Upptäckt av små GTPase Prenylation och GTP…
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Biochemistry
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JoVE Journal Biochemistry
Upptäckt av små GTPase Prenylation och GTP bindande använder membran fraktionering och GTPase-linked Immunosorbent Assay
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Upptäckt av små GTPase Prenylation och GTP bindande använder membran fraktionering och GTPase-linked Immunosorbent Assay

Article DOI: 10.3791/57646-v • 13:52 min • November 11th, 2018
November 11th, 2018 •
1,2, 1,2,3, 1, 1,2, 4, 5, 6,7,8, 1,9, 1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

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Här beskriver vi ett protokoll för att undersöka prenylation och guanosin-5'-trifosfat (GTP)-lastning av Rho GTPase. Detta protokoll består av två detaljerade metoder, nämligen membran fraktionering och en GTPase-linked immunosorbent assay. Protokollet kan användas för att mäta prenylation och GTP lastning av olika andra små GTPases.

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Biokemi fråga 141 Simvastatin glioblastom Rho GTPase cancer cellbiologi prenylation mevalonate väg
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