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Immunology and Infection

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JoVE Journal Immunology and Infection
Lys-ark mikroskopi for tre-dimensionelle visualisering af Human immun celler
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Lys-ark mikroskopi for tre-dimensionelle visualisering af Human immun celler

Article DOI: 10.3791/57651-v • 09:44 min • June 13th, 2018
June 13th, 2018 •
1, 1, 1, 1
1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine, Saarland University

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Vi præsenterer her, en protokol for at visualisere immunceller indlejret i en tre-dimensionelle (3D) kollagen matrix ved hjælp af lys-ark mikroskopi. Denne protokol også uddyber hvordan man spore celle migration i 3D. Denne protokol kan være ansat af andre former for affjedring celler i den 3D matrix.

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Immunologi og infektion sag 136 lys-ark mikroskopi enkelt fly belysning mikroskopi human immun celler natural killer celler cytotoksiske T-lymfocytter celle tracking 3D imaging levende celle imaging celle fiksering i kollagen
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