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JoVE Journal Cancer Research
Kendetegner DNA reparationsprocesser på forbigående og langvarig dobbelt-strenget DNA pauser ved immunfluorescens mikroskopi
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Kendetegner DNA reparationsprocesser på forbigående og langvarig dobbelt-strenget DNA pauser ved immunfluorescens mikroskopi

Article DOI: 10.3791/57653-v • 08:31 min • June 8th, 2018
June 8th, 2018 •
*1, *1, 2, 1, 1, 1, 1, 1, 1
1Division of Biology, Kansas State University, 2Bristol Medical School, Translational Health Sciences, University of Bristol
* These authors contributed equally

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Reparation af dobbelt-strenget DNA pauser er en dynamisk proces, der kræver ikke kun dannelsen af reparation komplekser på pauserne, men også deres beslutning efter læsionen er behandlet. Her bruger vi immunofluorescens mikroskopi for forbigående og langvarig dobbelt-strenget pauser som et redskab til at dissekere denne genom vedligeholdelse mekanisme.

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Kræftforskning sag 136 immunofluorescens mikroskopi DNA reparation dobbelt-strenget pauser reparation kinetik homologe rekombination ikke-homologe ende sammenføjning H2AX
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