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Karakterisere DNA reparasjon prosesser på forbigående og lang-varer dobbel-strand DNA bryter av Immunofluorescence mikroskopi
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Karakterisere DNA reparasjon prosesser på forbigående og lang-varer dobbel-strand DNA bryter av Immunofluorescence mikroskopi

Article DOI: 10.3791/57653-v • 08:31 min • June 8th, 2018
June 8th, 2018 •
*1, *1, 2, 1, 1, 1, 1, 1, 1
1Division of Biology, Kansas State University, 2Bristol Medical School, Translational Health Sciences, University of Bristol
* These authors contributed equally

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Reparasjon av dobbel-strand DNA bryter er en dynamisk prosess, krever ikke bare dannelsen av reparasjon komplekser pauser, men også deres oppløsning etter lesjonen er adressert. Her, bruker vi immunofluorescence mikroskopi forbigående og langvarig double-strandet pausene som et verktøy for å analysere denne genomet vedlikehold mekanismen.

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Kreftforskning problemet 136 Immunofluorescence mikroskopi DNA reparasjon dobbel-strand bryter reparasjon kinetikk homologe rekombinasjon ikke-homologe slutten begynte H2AX
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