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JoVE Journal Biochemistry
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Article DOI: 10.3791/57674-v • 12:26 min • May 3rd, 2018
May 3rd, 2018 •
*1, *1, 1, 1
1Department of Chemistry, State University of New York at Binghamton
* These authors contributed equally

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Summary
Automatic Translation

Cyclin-dependent kinase 1 (Cdk1) is activated in the G2 phase of the cell cycle and regulates many cellular pathways. Here, we present a protocol for an in vitro kinase assay with Cdk1, which allows the identification of Cdk1-specific phosphorylation sites for establishing cellular targets of this important kinase.

Tags

In Vitro Kinase Assay Cyclin-dependent Kinase 1 Phosphorylation Sites Protein Of Interest Cell Cycle Regulation Chromosome Segregation Chromosomal Aberrations Cancer Purified Proteins Model Organism Reliable Results Functional Studies CDK1 Targets Proteum Other Kinases Optimized Conditions Phosphorylation Efficiency IGPS 3.0 Software CENP-F Target Protein Sequence
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