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JoVE Journal
Biochemistry

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体外激酶法鉴定细胞周期蛋白依赖性激酶1特异磷酸化部位
 
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体外激酶法鉴定细胞周期蛋白依赖性激酶1特异磷酸化部位

Article DOI: 10.3791/57674
May 3rd, 2018

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Summary May 3rd, 2018

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细胞周期蛋白依赖性激酶 1 (Cdk1) 在 G2 阶段被激活并且调控许多细胞通路。在这里, 我们提出了一个协议的体外激酶检测与 Cdk1, 这使得识别 Cdk1-specific 磷酸化的网站, 以建立细胞目标的这个重要的激酶。

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