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Journal / Biochemistry / Identifiering av cyklin-beroende Kinase 1…
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Biochemistry

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JoVE Journal Biochemistry
Identifiering av cyklin-beroende Kinase 1 särskilda fosforylering platser av ett In Vitro -Kinas Assay
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
Journal (Biochemistry)
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
 
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Identifiering av cyklin-beroende Kinase 1 särskilda fosforylering platser av ett In Vitro -Kinas Assay

Article DOI: 10.3791/57674-v • 12:26 min • May 3rd, 2018
May 3rd, 2018 •
*1, *1, 1, 1
1Department of Chemistry, State University of New York at Binghamton
* These authors contributed equally

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Cyklin-beroende kinase 1 (Cdk1) aktiveras i den G2-fasen i cellcykeln och reglerar många cellulära vägar. Här presenterar vi ett protokoll för en in vitro- Kinas analys med Cdk1, som möjliggör identifiering av Cdk1-specifika fosforylering platser för fastställande av cellulära mål denna viktigt-Kinas.

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Biokemi fråga 135 Cyclin-beroende kinase 1 cellkärnelokalisering signal Kinas assay fosforylering masspektrometri analytisk gelfiltrering karyopherin alpha CENP-F G2 fas cellcykeln Kinas affinitet rening
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