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Developmental Biology

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JoVE Journal Developmental Biology
Undertrykkelse av Pro-fibrotiske signalering Potentiates faktor-mediert omprogrammering av mus embryonale fibroblaster til indusert Cardiomyocytes
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Undertrykkelse av Pro-fibrotiske signalering Potentiates faktor-mediert omprogrammering av mus embryonale fibroblaster til indusert Cardiomyocytes

Article DOI: 10.3791/57687-v • 09:16 min • June 3rd, 2018
June 3rd, 2018 •
1, 1, 1, 1, 2, 1
1Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, 2Department of Population Health Sciences, Medical College of Georgia, Augusta University

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Her presenterer vi en robust metode for å omprogrammere primære embryonale fibroblaster i funksjonelle cardiomyocytes gjennom overuttrykte GATA4, Hand2, Mef2c, Tbx5, miR-1 og miR-133 (GHMT2m) sammen med hemming av TGF-β signalering. Våre protokollen genererer slo cardiomyocytes så tidlig som i 7 dager etter signaltransduksjon med opptil 60% effektivitet.

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Utviklingspsykologi biologi problemet 136 Cardiac reprogramming transkripsjonsfaktorer sammensatte microRNAs pro-fibrotiske signalnettverk TGF-β reseptor 1 hemmer
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