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Multi-farve lokalisering mikroskopi af enkelt Membranproteiner i organeller af levende pattedyrceller
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Multi-farve lokalisering mikroskopi af enkelt Membranproteiner i organeller af levende pattedyrceller

Article DOI: 10.3791/57690-v • 11:06 min • June 30th, 2018
June 30th, 2018 •
1, 1, 1, 2, 1,3
1School of Biology, University of Osnabrück, 2Center of Cellular Nanoanalytics, Integrated Bioimaging Facility, University of Osnabrück, 3Department of Biology, WWU Münster

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Vi præsenterer her, en protokol for multi-farve lokalisering af enkelt Membranproteiner i organeller af levende celler. Du kan vedhæfte fluorophores, bruges selvstændig mærkning proteiner. Proteiner, beliggende i forskellige membraner rum af den samme organelle, kan lokaliseres med en nøjagtighed på ~ 18 nm.

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Biologi sag 136 enkelt molekyle tracking tofarvet super-resolution mikroskopi enkelt molekyle lokalisering rodamin farvestoffer mitokondrier dynamikken i Membranproteiner live celle imaging
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