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JoVE Journal Bioengineering
Un approccio basato su goccia di microfluidica e microsfere-PCR amplificazione per ampliconi di DNA Single-Stranded
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Un approccio basato su goccia di microfluidica e microsfere-PCR amplificazione per ampliconi di DNA Single-Stranded

Article DOI: 10.3791/57703-v • 11:40 min • November 14th, 2018
November 14th, 2018 •
*1, *2, 2, 3, 3, 1, 2,3, 1
1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University
* These authors contributed equally

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Questo lavoro fornisce un metodo per la realizzazione di piattaforme microfluidici basati su gocciolina e l'applicazione di microsfere di poliacrilammide per l'amplificazione di PCR di microsfere. Il metodo di microsfere-PCR permette di ottenere single-stranded DNA ampliconi senza separare DNA double-stranded.

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Bioingegneria problema 141 copolimerizzazione microfluidica canale PCR-flusso-messa a fuoco a microsfere ssDNA amplificazione PCR asimmetrica
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