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Bioengineering

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JoVE Journal Bioengineering
En dråpe Microfluidic tilnærming og Microsphere PCR forsterkning for Single-strandet DNA Amplicons
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En dråpe Microfluidic tilnærming og Microsphere PCR forsterkning for Single-strandet DNA Amplicons

Article DOI: 10.3791/57703-v • 11:40 min • November 14th, 2018
November 14th, 2018 •
*1, *2, 2, 3, 3, 1, 2,3, 1
1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University
* These authors contributed equally

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Dette arbeidet gir en metode for fabrikasjon av droplet-baserte microfluidic plattformer og anvendelse av polyakrylamid mikrosfærer for microsphere PCR forsterkning. Microsphere PCR-metoden gjør det mulig å få enkelt-strandet DNA amplicons uten skille double-strandet DNA.

Tags

Bioteknologi problemet 141 Copolymerization Microfluidic kanalen flyt-fokus Microsphere PCR ssDNA forsterkning asymmetrisk PCR
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