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JoVE Journal
Developmental Biology

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果蝇分子和免疫组化分析制备周围嗅组织的研制
 
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果蝇分子和免疫组化分析制备周围嗅组织的研制

Article DOI: 10.3791/57716-v 09:18 min June 13th, 2018
June 13th, 2018

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Summary

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在这里, 我们提出了一个阶段和解剖发展的嗅觉组织从果蝇物种的协议。解剖后的组织可用于分子分析, 如定量 rt-pcr (反向转录-聚合酶链反应) 或 RNA 测序 (RNAseq), 以及体内分析, 如免疫组化或原位杂交。

Tags

发育生物学 问题 136,果蝇种类 嗅觉系统开发 触角盘 触角 解剖 转录分析
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