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JoVE Journal Developmental Biology
Forbereder utvikle perifere Olfactory vev for molekylær og Immunohistochemical analyse i Drosophila
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Forbereder utvikle perifere Olfactory vev for molekylær og Immunohistochemical analyse i Drosophila

Article DOI: 10.3791/57716-v • 09:18 min • June 13th, 2018
June 13th, 2018 •
1, 1
1Department of Biology, Duke University

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Her presenterer vi en protokoll for scenen og dissekere utvikle olfactory vev fra Drosophila arter. Dissekert vevet kan senere brukes til molekylær analyser, som kvantitative RT PCR (omvendt transkripsjon-polymerasekjedereaksjons) eller RNA sekvensering (RNAseq), samt i vivo analyser som immunohistochemistry eller i situ hybridisering.

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Utviklingspsykologi biologi problemet 136 Drosophila arter olfactory systemutvikling antennal plate antenner disseksjoner transcriptional profilering
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