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Ett flöde flödescytometri-baserad analys för att mäta mitokondriella membranet Potential i hjärt myocyter efter hypoxi/Reoxygenation
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Ett flöde flödescytometri-baserad analys för att mäta mitokondriella membranet Potential i hjärt myocyter efter hypoxi/Reoxygenation

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Här presenterar vi ett protokoll som använder JC-1 färgämne för att utvärdera mitokondriella membranet potential celler efter att utsättas för hypoxi/reoxygenation med eller utan en skyddande agent.

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Beteende fråga 137 mitokondriell permeabilitet övergången pore mitokondriella membranpotential JC-1 assay reperfusionsskada hjärt myocyter flödescytometri
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