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Journal / Genetics / Lavado broncoalveolar exosomas en lesión pulmonar …
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Genetics

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JoVE Journal Genetics
Lavado broncoalveolar exosomas en lesión pulmonar séptica inducida por lipopolisacárido
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Journal (Genetics)
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Lavado broncoalveolar exosomas en lesión pulmonar séptica inducida por lipopolisacárido

Article DOI: 10.3791/57737-v • 08:27 min • May 21st, 2018
May 21st, 2018 •
1,2, 1, 1,2
1Department of Veterans Affairs, Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

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Ratones expuestos a LPS intraperitoneales secretan exosomas en el líquido del lavado (BAL) Bronco-alveolar que se empaquetan con miRNAs. Utiliza un sistema de cocultivo, demostramos que los exosomas liberados en el líquido del BAL interrumpen la expresión de proteínas de Unión estrecha en células epiteliales bronquiales y aumentan la expresión de citoquinas pro-inflamatorias que acentúan la lesión pulmonar.

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Genética número 135 exosomas microRNAs miR-155 miR-146a LPS pulmonar sepsis
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