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用 X 射线晶体学和生物物理技术表征免疫球蛋白折叠的糖蛋白
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Biochemistry
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JoVE Journal Biochemistry
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
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用 X 射线晶体学和生物物理技术表征免疫球蛋白折叠的糖蛋白

DOI:
10.3791/57750-v

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08:58 min

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July 05, 2018

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, , , ,
1Program in Molecular Medicine,The Hospital for Sick Children Research Institute, 2Department of Biochemistry,University of Toronto, 3Department of Immunology,University of Toronto

Chapters

  • 00:05Title
  • 00:38Purification of Soluble Glycoprotein from HEK293 Supernatant
  • 02:08Crystallization of Glycoproteins
  • 03:44Soaking Glycoprotein Crystals with its Ligand
  • 04:33Characterization of Fab and Small Molecule Binding to the Glycoprotein
  • 07:34Results: Characterization of Glycoproteins
  • 08:24Conclusion

Summary

Automatic Translation

Automatic Translation

通过 biolayer 干涉法、等温滴定量热仪和 X 射线晶体学, 我们提出了免疫球蛋白褶皱的生物物理和结构表征方法。

Tags

GlycoproteinsImmunoglobulin FoldX-ray CrystallographyBiophysical TechniquesProtein FunctionN-linked GlycansAntibioticsStructural CharacterizationProtein PurificationSize Exclusion ChromatographyDeglycosylationProtein ConcentrationCrystallization

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