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JoVE Journal Neuroscience
Optimalisering av Laser-fangst Microdissection for isolering av innstigning Ganglion fra frisk-frosne menneskelig vev
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Optimalisering av Laser-fangst Microdissection for isolering av innstigning Ganglion fra frisk-frosne menneskelig vev

Article DOI: 10.3791/57762-v • 08:28 min • June 14th, 2018
June 14th, 2018 •
1, 1, 1
1Vanderbilt University Medical Center

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Målet med denne protokollen er å oppnå høy integritet RNA prøver fra innstigning Ganglion isolert fra unfixed, fersk resected menneskelige tarmen tissue bruker laser fange microdissection (LCM). Denne protokollen involverer forberede flash-frosne prøver av menneskelige tarmen tissue, og kryosnitt, ethanolic flekker og dehydrering, LCM, og RNA utvinning.

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Nevrovitenskap problemet 136 Laser-fangst microdissection (LCM) menneskelige tarmen tissue tarmen enteric Ganglion enteric nervesystemet RNA utvinning Cresyl Violet flekker RNA sekvensering (RNA-Seq)
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