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JoVE Journal Neuroscience
Optimering af Laser-Capture Microdissection til isolering af entero ganglier fra frisk frosset menneskeligt væv
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Optimering af Laser-Capture Microdissection til isolering af entero ganglier fra frisk frosset menneskeligt væv

Article DOI: 10.3791/57762-v • 08:28 min • June 14th, 2018
June 14th, 2018 •
1, 1, 1
1Vanderbilt University Medical Center

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Målet med denne protokol er at få høj-integritet RNA prøver fra enterisk ganglier isoleret fra ikke optagne, frisk resektion menneskelige tarmens væv ved hjælp af laser fange microdissection (LCM). Denne protokol omfatter flash-frosset prøver af menneskelige tarmens væv, cryosectioning, ethanoliske farvning og dehydrering, LCM, og RNA udvinding.

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Neurovidenskab sag 136 Laser-capture microdissection (LCM) menneskelige tarmens væv tarmen entero ganglier enteriske nervesystem RNA udvinding Cresyl Violet farvning RNA sekventering (RNA-Seq)
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