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Journal / Neuroscience / FM farvestof cykling på Synapse: sammenligning af …
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JoVE Journal Neuroscience
FM farvestof cykling på Synapse
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FM farvestof cykling på Synapse: sammenligning af høje kalium depolarisering, elektriske og Channelrhodopsin Stimulation

Article DOI: 10.3791/57765-v • 08:31 min • May 24th, 2018
May 24th, 2018 •
1, 2
1Department of Biological Sciences, Vanderbilt University, 2Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development, Vanderbilt University and Medical Center

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Synaptic vesikel (SV) cykling er den centrale mekanisme for intercellulær kommunikation på neuronal synapser. FM farvestof optagelse og udgivelse er det primære middel til kvantitativt boltpistoler SV endo- og exocytose. Her, sammenligner vi alle stimulation metoderne til at drive FM1-43 cykling på Drosophila neuromuskulære junction (NMJ) model synapse.

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Neurovidenskab spørgsmålet 135 Drosophila neuromuskulære junction FM1-43 Elektrofysiologi photoconversion transmissions elektronmikroskopi
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