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Journal / Immunology and Infection / Identifikasjon av virulens markører av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikasjon av virulens markører av Mycobacterium abscessus for intracellulær replikering i Phagocytes
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Identifikasjon av virulens markører av Mycobacterium abscessus for intracellulær replikering i Phagocytes

Article DOI: 10.3791/57766-v • 08:34 min • September 27th, 2018
September 27th, 2018 •
*1, *1, 1, 1, 2, 1, 1
12I, UVSQ, INSERM UMR1173, Université Paris-Saclay, 2Institut Pasteur, Unité de Pathogénomique Mycobactérienne
* These authors contributed equally

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Her presenterer vi to protokoller for å studere Phagocyte -Mycobacterium abscessus vekselsvirkningene: screening av et transposon mutant bibliotek for bakteriell intracellulær mangel og fastsettelse av bakteriell intracellulær transcriptome fra RNA sekvensering. Begge metodene gir innsikt i genomisk fordeler og transcriptomic tilpasninger styrke intracellulære bakterier fitness.

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Immunologi og infeksjon problemet 139 mikrobiologi amoeba Acanthamoeba castellanii Mycobacterium abscessus transposon biblioteket co kultur amoeba-mykobakterier RNA utvinning av intracellulær mykobakterier RNAseq.
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