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Journal / Immunology and Infection / Encellede kvantifisering av mRNA og overflaten…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Encellede kvantifisering av mRNA og overflaten Protein uttrykk i Simian immunsvikt-Virus-infiserte CD4+ T celler isolert fra Rhesus aper
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Encellede kvantifisering av mRNA og overflaten Protein uttrykk i Simian immunsvikt-Virus-infiserte CD4+ T celler isolert fra Rhesus aper

Article DOI: 10.3791/57776-v • 13:13 min • September 25th, 2018
September 25th, 2018 •
1, 1, 1, 2, 1
1US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID, NIH

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Beskrevet er en metode for å quantitate uttrykket av 96 gener 18 overflaten proteiner av enkeltceller ex vivo, tillater for identifikasjon av ulikt uttrykt gener og proteiner i virus-infiserte celler i forhold til uninfected celler. Vi bruker tilnærming til studiet SIV-infiserte CD4+ T celler isolert fra rhesus aper.

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Immunologi og infeksjon problemet 139 enkeltcelle SIV mRNA multiplekset qPCR indekserte flowcytometri rhesus aper
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