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Journal / Immunology and Infection / 猕猴免疫缺陷病毒感染 CD4+ T 细胞单细胞定量的 mRNA…
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Immunology and Infection

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JoVE Journal Immunology and Infection
猕猴免疫缺陷病毒感染 CD4+ T 细胞单细胞定量的 mRNA 和表面蛋白的表达
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Journal (Immunology and Infection)
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Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
Journal (Immunology and Infection)
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猕猴免疫缺陷病毒感染 CD4+ T 细胞单细胞定量的 mRNA 和表面蛋白的表达

Article DOI: 10.3791/57776-v • 13:13 min • September 25th, 2018
September 25th, 2018 •
1, 1, 1, 2, 1
1US Military HIV Research Program, Henry M. Jackson Foundation, Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID, NIH

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描述的方法, 以定量的表达96基因和18表面蛋白的单细胞前体, 允许鉴定差异表达基因和蛋白质的病毒感染细胞相对未感染的细胞。我们采用这种方法来研究与恒河猴分离的 SIV 感染的 CD4+ T 细胞。

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免疫学和感染 问题 139 单细胞 SIV mRNA 复用 qPCR 索引流式细胞仪 恒河猴
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