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Journal / Developmental Biology / Induktion og validering af cellulære gulne i…
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Developmental Biology

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JoVE Journal Developmental Biology
Induktion og validering af cellulære gulne i primære humane celler
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Induktion og validering af cellulære gulne i primære humane celler

Article DOI: 10.3791/57782-v • 08:18 min • June 20th, 2018
June 20th, 2018 •
1, 1, 1
1European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen

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Her diskuterer vi en række protokoller for induktion og validering af cellulære gulne i kulturperler celler. Vi fokuserer på forskellige gulne-inducerende stimuli og beskrive kvantificering af fælles gulne-associerede markører. Vi leverer tekniske detaljer ved hjælp af fibroblaster som model, men protokollerne, der kan tilpasses til forskellige cellulære modeller.

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Udviklingsmæssige biologi spørgsmålet 136 cellulære gulne aldring kræft tumor undertrykkelse væv reparation sekretion Gen-ekspression genotoksiske stress kemoterapi bestråling Epigenetik
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