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JoVE Journal Developmental Biology
Een vereenvoudigde en efficiënte methode om te isoleren van de primaire mens keratinocyten van volwassen huidweefsel
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Een vereenvoudigde en efficiënte methode om te isoleren van de primaire mens keratinocyten van volwassen huidweefsel

Article DOI: 10.3791/57784-v • 06:17 min • August 25th, 2018
August 25th, 2018 •
1, 1,2, 1, 1, 3, 1, 1,2
1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology, Qilu Hospital of Shandong University

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Hier presenteren we een protocol om efficiënt isoleren primaire menselijke keratinocyten van volwassen huid weefsels. Deze methode vereenvoudigt de conventionele procedure met behulp van de Y-27632 van de Inhibitor van de omwenteling ROCK in het medium van de inoculatie spontaan epidermale cellen uit dermale cellen scheiden.

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Ontwikkelingsbiologie kwestie 138 menselijke keratinocyten epidermale cellen van de stam dermale cellen primaire huid cel isolatie Keratinocyt cultuur Y-27632 Rho-geassocieerde kinase inhibitor regeneratie van de huid
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