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Developmental Biology

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JoVE Journal Developmental Biology
Traceren van genexpressie door detectie van β-galactosidase-activiteit in hele muis embryo 's
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Traceren van genexpressie door detectie van β-galactosidase-activiteit in hele muis embryo 's

Article DOI: 10.3791/57785-v • 08:42 min • June 26th, 2018
June 26th, 2018 •
*1, *1, 1, 1, 1, 1, 2,3
1Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research, Universidad Europea de Madrid
* These authors contributed equally

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Hier beschrijven we het standaardprotocol voor de detectie van β-galactosidase-activiteit in vroege hele muis embryo's en de methode voor paraffine segmenteren en counterstaining. Dit is een gemakkelijke en snelle procedure om te controleren van genexpressie tijdens de ontwikkeling die ook kan worden toegepast op weefselsecties, organen of gekweekte cellen.

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Ontwikkelingsbiologie kwestie 136 LacZ β-galactosidase paraffine afdelen muis ontwikkeling embryonale verduidelijking genexpressie
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