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Journal / Genetics / 基于生物素的下拉法对细胞 miRNAs mRNA…
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JoVE Journal Genetics
基于生物素的下拉法对细胞 miRNAs mRNA 靶点的验证
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基于生物素的下拉法对细胞 miRNAs mRNA 靶点的验证

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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本报告描述了一种快速可靠的方法来验证细胞 miRNAs 的 mRNA 目标。该方法采用合成生物素化锁定核酸 (低噪声放大) miRNA 模拟捕获靶 mRNA。随后, 采用链亲和素包覆磁性微珠对靶 mRNA 进行 qPCR 聚合酶链反应的定量。

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遗传学 问题 136 miRNA mRNA 低噪声放大 biotinylation PCR 3 ' UTR
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