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Journal / Genetics / Biotin-baserede Pulldown Assay til Valider mRNA…
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Genetics

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JoVE Journal Genetics
Biotin-baserede Pulldown Assay til Valider mRNA mål af cellulære miRNAs
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Biotin-baserede Pulldown Assay til Valider mRNA mål af cellulære miRNAs

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Denne rapport beskriver en hurtig og pålidelig metode til validering af cellulære miRNAs mRNA mål. De metode bruger syntetiske biotinylated låst nukleinsyre LNA-baserede miRNA efterligner for at fange målrette mRNA. Efterfølgende, streptavidin-belagt magnetiske perler er ansat til pulldown målet mRNA for kvantificering af qPCR polymerase kæde reaktion.

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Genetik sag 136 miRNA mRNA LNA biotinylation PCR 3' UTR
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