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Journal / Genetics / Biotin-baserade Pulldown Assay att validera mRNA…
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Genetics

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JoVE Journal Genetics
Biotin-baserade Pulldown Assay att validera mRNA mål av cellulära MicroRNA
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Biotin-baserade Pulldown Assay att validera mRNA mål av cellulära MicroRNA

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Denna rapport beskriver en snabb och tillförlitlig metod för att validera mRNA mål av cellulära microRNA. De metoden använder syntetiska biotinylerade låst nukleinsyra LNA-baserade miRNA härmar för att fånga rikta mRNA. Därefter streptividin-belagda magnetiska pärlor är anställda att pulldown målet mRNA för kvantifiering av qPCR polymeras-kedjereaktion.

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Genetik fråga 136 miRNA mRNA LNA biotinylation PCR 3' UTR
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