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Cancer Research

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JoVE Journal Cancer Research
Análise de sequência do Gene da imunoglobulina na leucemia linfocítica crônica
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Análise de sequência do Gene da imunoglobulina na leucemia linfocítica crônica: Do paciente Material para interpretação de sequência

Article DOI: 10.3791/57787-v • 09:02 min • November 26th, 2018
November 26th, 2018 •
*1, *2,3, 1, 4, 5, 6, 7, 2,3, 1,2, 8
1Institute of Applied Biosciences, Centre for Research and Technology Hellas, 2Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, 3Department of Molecular Medicine and Surgery, Karolinska Institutet, 4Division of Immunology, Transplantation and Infectious, IRCCS San Raffaele Scientific Institute, 5Department of Immunology, Laboratory for Medical Immunology, Erasmus University Medical Center, 6Hematology Department, Nikea General Hospital, 7Assistance publique - Hôpitaux de Paris (AP-HP), Hopital Pitié-Salpêtrière, Department of Hematology, and UPMC University Paris 06, UMRS 1138, 8Division of Experimental Oncology, IRCCS Istituto Scientifico San Raffaele and Università Vita-Salute San Raffaele
* These authors contributed equally

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Neste documento, apresentamos um protocolo que detalhes os aspectos técnicos e requisitos essenciais para garantir a análise de sequências de genes IG robusto em pacientes com leucemia linfocítica crônica (CLL), baseado na experiência acumulada da iniciativa de investigação europeia CLL (ERIC).

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Cancer Research edição 141 leucemia linfocítica crônica (CLL) somática (SHM) imunoglobulina variável pesadas de imunoglobulina (IGHV) complementaridade determinando a região 3 (CDR3) sistema de informação internacional Imunogenética (IMGT)
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