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JoVE Journal Neuroscience
Fette di cervello-ipofisi sane per le indagini elettrofisiologiche delle cellule ipofisarie in pesci teleostei
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Fette di cervello-ipofisi sane per le indagini elettrofisiologiche delle cellule ipofisarie in pesci teleostei

Article DOI: 10.3791/57790-v • 07:14 min • August 16th, 2018
August 16th, 2018 •
*1, *1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences
* These authors contributed equally

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L'articolo descrive un protocollo ottimizzato per fare fette di tessuto vitale del cervello-ipofisi, utilizzando il medaka pesci teleostei (Oryzias latipes), seguita da registrazioni elettrofisiologiche delle cellule ipofisarie utilizzando la tecnica del patch-clamp con il configurazione di patch perforato.

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Neuroscienze problema 138 cervello-ipofisi tessuto-fetta elettrofisiologia pesce patch-clamp cellule endocrine
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