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Biochemistry

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JoVE Journal Biochemistry
Purification et Reconstitution de TRPV1 pour analyse spectroscopique
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Purification et Reconstitution de TRPV1 pour analyse spectroscopique

Article DOI: 10.3791/57796-v • 11:53 min • July 3rd, 2018
July 3rd, 2018 •
1, 2, 1,3, 1, 1
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 3CuriRX, Inc.

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Cet article décrit des méthodes spécifiques pour obtenir des quantités biochimiques de TRPV1 solubilisée détergent pour analyse spectroscopique. Les protocoles combinés offrent des outils biochimiques et biophysiques qui peuvent être adaptées afin de faciliter les études structurales et fonctionnelles des canaux ioniques mammifères dans un environnement contrôlé en membrane.

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Biochimie numéro 137 Transient receptor potentiels VANILLOÏDE 1 TRPV1 purification reconstitution spin-étiquetage spectroscopie RPE spectroscopie de cerf
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