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JoVE Journal Biochemistry
Zuivering en reconstructie van TRPV1 spectroscopische p.a.
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Zuivering en reconstructie van TRPV1 spectroscopische p.a.

Article DOI: 10.3791/57796-v • 11:53 min • July 3rd, 2018
July 3rd, 2018 •
1, 2, 1,3, 1, 1
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 3CuriRX, Inc.

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Dit artikel wordt beschreven specifieke methoden voor biochemische hoeveelheid wasmiddel-ontbindend TRPV1 spectroscopische p.a.. De gecombineerde protocollen bieden biochemische en biofysische hulpmiddelen die kunnen worden aangepast om de structurele en functionele studies voor zoogdieren ionenkanalen in een membraan-gecontroleerde omgeving.

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Biochemie kwestie 137 voorbijgaande receptor potentiële vanilloid 1 TRPV1 zuivering reconstructie spin-labeling EPR spectroscopie herten spectroscopie
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