Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

Yashar Bashirzadeh; Siddharth Chatterji; Dakota Palmer; Sandeep Dumbali; Shizhi Qian; Venkat Maruthamuthu

doi:10.3791/57797

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Stivhed måling af blød silikone underlag for Mechanobiology undersøgelser ved hjælp af en Widefield fluorescens mikroskop

Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

Stivhed måling af blød silikone underlag for Mechanobiology undersøgelser ved hjælp af en Widefield fluorescens mikroskop

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6,767 Views

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07:02 min

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July 03, 2018

DOI:

10.3791/57797-v

DOI:

10.3791/57797-v

•

07:02 min

July 03, 2018

•

3 Views

Yashar Bashirzadeh*¹, Siddharth Chatterji*¹, Dakota Palmer*², Sandeep Dumbali*¹, Shizhi Qian¹, Venkat Maruthamuthu¹

¹Department of Mechanical & Aerospace Engineering,Old Dominion University, ²Department of Biological Sciences,Old Dominion University

Transcript

Summary

Substrater med stivhed i kilopascal-området er nyttige at studere svar af celler til fysiologisk relevante mikro-miljø stivhed. Bruger bare en widefield fluorescens mikroskop, kan de unge modulus af blød silikone gel bestemmes ved hjælp af en indrykning med en egnet kugle.