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JoVE Journal
Biochemistry

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Effektiv rensning og LC-MS/MS-baseret analyse udvikling for ti-elleve omplantning-2 5-Methylcytosine Dioxygenase
 
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Effektiv rensning og LC-MS/MS-baseret analyse udvikling for ti-elleve omplantning-2 5-Methylcytosine Dioxygenase

Article DOI: 10.3791/57798-v 10:34 min October 15th, 2018
October 15th, 2018

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Summary

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Vi præsenterer her, en protokol for en effektiv enkelt trin rensning af den aktive ukodede menneskelige ti-elleve omplantning-2 (TET2) 5-methylcytosine dioxygenase ved hjælp af ion-udveksling kromatografi og sin analyse ved hjælp af en liquid chromatography-tandem masse massespektrometri (LC-MS/MS)-baseret tilgang.

Tags

Biokemi spørgsmål 140 ti-elleve translokation Demethylase leukæmi Dioxygenase LC-MS/MS Epigenetik transskription forordning
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