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Journal / Biochemistry / Effektiv rensing og LC-MS/MS-baserte analysen…
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Biochemistry

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JoVE Journal Biochemistry
Effektiv rensing og LC-MS/MS-baserte analysen utvikling for ti-elleve translokasjon-2 5-Methylcytosine Dioxygenase
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Effektiv rensing og LC-MS/MS-baserte analysen utvikling for ti-elleve translokasjon-2 5-Methylcytosine Dioxygenase

Article DOI: 10.3791/57798-v • 10:34 min • October 15th, 2018
October 15th, 2018 •
*1, *1, *1, 1, 1
1Division of Pharmaceutical Sciences, School of Pharmacy, University of Missouri-Kansas City
* These authors contributed equally

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Her presenterer vi en protokoll for en effektiv enkeltsteg rensing av den aktive ukodede menneskelige ti-elleve translokasjon-2 (TET2) 5-methylcytosine dioxygenase ion-exchange kromatografi og dens analysen med en flytende kromatografi-tandem masse massespektrometri (LC-MS/MS)-tilnærming.

Tags

Biokjemi problemet 140 ti-elleve translokasjon Demethylase leukemi Dioxygenase LC--MS-/ MS Epigenetics transkripsjon regulering
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