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Biochemistry

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JoVE Journal Biochemistry
Kleinschalige colorimetrische testen van intracellulaire lactaat en pyruvaat in de Nematode Caenorhabditis elegans
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Kleinschalige colorimetrische testen van intracellulaire lactaat en pyruvaat in de Nematode Caenorhabditis elegans

Article DOI: 10.3791/57807-v • 09:35 min • October 15th, 2018
October 15th, 2018 •
1,2, 3, 3
1Department of Health Science, School of Sports & Health Science, Daito Bunka University, 2Department of Molecular Life Science, School of Medicine, Tokai University, 3Department of Health Management, School of Health Study, Tokai University

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We beschrijven een gemodificeerde kleinschalige extractie en colorimetrische vitrotests van lactaat en pyruvaat in de nematode C. elegans. Bij gebruik van commerciële assay kits, is de technische ontwikkeling van hun gevoeligheid en nauwkeurigheid belangrijk. Eiwit neerslag in extractie is de meest kritische stap voor de kwantitatieve bepaling van intracellulaire metabolieten.

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Biochemie kwestie 140 Caenorhabditis elegans energiemetabolisme lactaat pyruvaat gemeenschappelijke precipitant colorimetrische bepaling
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