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En roman metning mutagenese tilnærming
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En roman metning mutagenese tilnærming: Enkeltsteg karakteristikk av regulatoriske Protein bindende områder i RNA bruker Phosphorothioates

Article DOI: 10.3791/57816-v • 11:49 min • August 21st, 2018
August 21st, 2018 •
1
1Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder

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Proteiner som binder bestemt RNA sekvenser spille viktige roller i genuttrykk. Detaljert karakterisering av disse bindende områder er avgjørende for vår forståelse av genet regulering. Her beskrives et enkeltsteg tilnærming for metning mutagenese av protein-bindende RNA. Denne tilnærmingen er relevant for alle protein-bindende områder i RNA.

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Genetikk problemet 138 RNA-bindende protein DNA-bindende protein phosphorothioates metning mutagenese kile-gel geleelektroforese autoradiography doping
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