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JoVE Journal Genetics
En kombinatorisk encellede tilgang til at karakterisere de molekylære og Immunophenotypic heterogenitet af menneskelige stamceller og stamfader populationer
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En kombinatorisk encellede tilgang til at karakterisere de molekylære og Immunophenotypic heterogenitet af menneskelige stamceller og stamfader populationer

Article DOI: 10.3791/57831-v • 09:34 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Division of Molecular Hematology, Lund Stem Cell Center, Lund University

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Bulk gen expression målinger cloud individuelle celle forskelle i heterogene cellepopulationer. Her, vi beskriver en protokol for hvordan encellede gen expression analyse og indeks sortering af fluorescens aktiveret celle sortering (FACS) kan kombineres for at afgrænse heterogenitet og immunophenotypically karakteriserer molekylemæssigt forskellige cellepopulationer.

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Genetik spørgsmål 140 heterogenitet encellede RT-qPCR FACS Index-sortering immunophenotypic karakterisering gene expression analyse
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