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Journal / Biochemistry / 哺乳动物 Bestrophin…
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Biochemistry

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JoVE Journal Biochemistry
哺乳动物 Bestrophin 离子通道的表达与纯化
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哺乳动物 Bestrophin 离子通道的表达与纯化

Article DOI: 10.3791/57832-v • 08:12 min • August 2nd, 2018
August 2nd, 2018 •
1, 1, 1, 1, 1
1Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry

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离子通道的纯化往往具有挑战性, 但一旦达到, 它就有可能允许对通道的功能和结构进行体外调查。在这里, 我们描述的步骤, 以表达和纯化哺乳动物 bestrophin 蛋白, 一个家庭的 Ca2 +激活 Cl 通道.

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生物化学 问题 138 哺乳动物膜蛋白 离子通道 视网膜退行性疾病 蛋白表达 蛋白质纯化 Bestrophin-1 BEST1 钙2 +活化氯离子通道 (CaCC)
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