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Analisi di co-immunoprecipitazione utilizzando proteine endogene nucleari da cellule coltivate in condizioni di ipossia
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Analisi di co-immunoprecipitazione utilizzando proteine endogene nucleari da cellule coltivate in condizioni di ipossia

Article DOI: 10.3791/57836-v • 09:17 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 1, 3, 4, 5, 3, 1,2,3, 1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute (SERI), Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 4Cancer and Stem Cell Biology Program, Duke-NUS Medical School, 5Department of Cell and Molecular Biology, Karolinska Institutet

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Qui descriviamo un protocollo di co-immunoprecipitazione per studiare le interazioni proteina-proteina tra proteine endogene nucleari in condizioni di ipossia. Questo metodo è adatto per la dimostrazione delle interazioni tra fattori di trascrizione e co-regolatori trascrizionali all'ipossia.

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Biologia problema 138 biologia molecolare co-immunoprecipitazione interazioni proteina-proteina (PPIs) ipossia fattori inducibili dall'ipossia (HIFs) proteine endogene estratti nucleari
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