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Co-immunoprecipitation Assay med endogena nukleära proteiner från celler odlade hypoxisk villkor
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Co-immunoprecipitation Assay med endogena nukleära proteiner från celler odlade hypoxisk villkor

Article DOI: 10.3791/57836-v • 09:17 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 1, 3, 4, 5, 3, 1,2,3, 1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute (SERI), Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 4Cancer and Stem Cell Biology Program, Duke-NUS Medical School, 5Department of Cell and Molecular Biology, Karolinska Institutet

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Här beskriver vi ett co-immunoprecipitation protokoll för att studera protein-protein interaktioner mellan endogena nukleära proteiner hypoxisk villkor. Denna metod är lämplig för demonstration av samspelet mellan transkriptionsfaktorer och transkriptionell samtidig regulatorer vid hypoxi.

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Biologi fråga 138 molekylärbiologi co-immunoprecipitation protein-protein interaktioner (PPI) hypoxi hypoxi-inducerbara faktorer (HIFs) endogena proteiner nukleära extrakt
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