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Co-immunoprecipitation analyse ved hjælp af endogene nukleare proteiner fra celler kulturperler hypoksiske betingelser
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Co-immunoprecipitation analyse ved hjælp af endogene nukleare proteiner fra celler kulturperler hypoksiske betingelser

Article DOI: 10.3791/57836-v • 09:17 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 1, 3, 4, 5, 3, 1,2,3, 1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute (SERI), Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 4Cancer and Stem Cell Biology Program, Duke-NUS Medical School, 5Department of Cell and Molecular Biology, Karolinska Institutet

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Her beskriver vi en co-immunoprecipitation protokol for at studere protein-protein interaktioner mellem endogene nukleare proteiner hypoksiske betingelser. Denne metode er velegnet til demonstration af vekselvirkninger mellem transkriptionsfaktorer og transcriptional Co lovgivere på hypoxi.

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Biologi spørgsmålet 138 Molekylærbiologi co-immunoprecipitation protein-protein interaktioner (ppi) hypoxi hypoxi-inducerbar faktorer (HIFs) endogene proteiner nukleare ekstrakter
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