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Biology

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Bestemmelse af Plasma membran partitionering for perifert forbundet proteiner
 
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Bestemmelse af Plasma membran partitionering for perifert forbundet proteiner

Article DOI: 10.3791/57837-v 11:11 min June 15th, 2018
June 15th, 2018

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Summary

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Vi præsenterer her, en protokol for at udføre en kvantitativ analyse af niveauet af plasma-membran association for fluorescently markeret perifert forbundet protein. Metoden er baseret på den beregningsmæssige nedbrydning af membranen og cytoplasmatisk komponent af signal observeret i celler mærket med plasma membran fluorescerende markør.

Tags

Biologi sag 136 perifert forbundet plasmamembran protein membran affinitet membran partitionering Konfokal mikroskopi ImageJ R-projektet FM 4-64 deconvolution
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