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Journal / Immunology and Infection / Karakterisering af Thymus-afhængige og…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Karakterisering af Thymus-afhængige og Thymus-uafhængig Immunoglobulin Isotype svar i mus ved hjælp af enzym-forbundet Immunosorbent Assay
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Karakterisering af Thymus-afhængige og Thymus-uafhængig Immunoglobulin Isotype svar i mus ved hjælp af enzym-forbundet Immunosorbent Assay

Article DOI: 10.3791/57843-v • 06:41 min • September 7th, 2018
September 7th, 2018 •
1, 1, 2,3
1Department of Cell Biology and Neuroscience and Graduate Program in Cellular and Molecular Pharmacology, Rutgers University, 2Department of Cell Biology and Neuroscience, Rutgers University, 3Rutgers Cancer Institute of New Jersey

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I dette papir beskriver vi en protokol for at karakterisere T-afhængige og T-uafhængig immunglobulin (Ig) isotype svar i mus ved hjælp af ELISA. Denne metode anvendes alene eller i kombination med flow flowcytometri giver forskere til at identificere forskelle i B-celle-medieret Ig isotypen svar i mus efter T-afhængige og T-uafhængig antigen immunisering.

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Immunologi og infektion sag 139 Thymus-afhængige (TD) antigen thymus-uafhængig (TI) antigen immunglobulin (Ig) Ig isotypen hukommelse svar immunisering adjuvans enzymmaerket assay (ELISA)
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