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JoVE Journal Bioengineering
En rask bildebasert bakteriell virulens analysen bruker Amoeba
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En rask bildebasert bakteriell virulens analysen bruker Amoeba

Article DOI: 10.3791/57844-v • 05:22 min • June 27th, 2018
June 27th, 2018 •
1, 1,2
1Department of Physics and Astronomy, University of California, 2Department of Molecular Biology and Biochemistry, University of California

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Her presenterer vi en protokoll for å måle virulens planktoniske eller overflate-vedlagt bakterier bruker D. discoideum (amoeba) som vert. Virulens måles over en periode på 1 time og vert drepe er kvantifisert fluorescens mikroskopi og bilde analyse. Vi viser denne bakterien P. aeruginosa-protokoll.

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Bioteknologi problemet 136 Pseudomonas aeruginosa overflate-vedlagt Planktonic Dictyostelium discoideum rask virulens analysen virulens i biofilm
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